Identifying hybridization and admixture using SNPs: Application of the DArTseq platforminphylogeographic research on vertebrates

J Melville; ML Haines; K Boysen; L Hodkinson; A Kilian; KL Smith Date; DA Potvin; KM Parris

Journal article

Identifying hybridization and admixture using SNPs: Application of the DArTseq platforminphylogeographic research on vertebrates

J Melville, ML Haines, K Boysen, L Hodkinson, A Kilian, KL Smith Date, DA Potvin, KM Parris

Royal Society Open Science | ROYAL SOC | Published : 2017

DOI: 10.1098/rsos.161061

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Abstract

Next-generation sequencing (NGS) approaches are increasingly being used to generate multi-locus data for phylogeographic and evolutionary genetics research. We detail the applicability of a restriction enzyme-mediated genome complexity reduction approach with subsequent NGS (DArTseq) in vertebrate study systems at different evolutionary and geographical scales. We present two case studies using SNP data from the DArTseq molecular marker platform. First, we used DArTseq in a large phylogeographic study of the agamid lizard Ctenophorus caudicinctus, including 91 individuals and spanning the geographical range of this species across arid Australia. A low-density DArTseq assay resulted in 28 960..

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University of Melbourne Researchers

Kirsten Parris Author

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Grants

Awarded by Australian Research Council

Funding Acknowledgements

Research was funded by the Australian Research Council (LP100200158, LP0990161, LP0667815) to J.M. and K.M.P. and The National Environmental Research Program, Environmental Decisions Hub to K.M.P.